Journal of neurochemistry 24:479-485.




A detailed time study of the incorporation of label from sodium-[1-14C]acetate, [1-14C]ethanol, and [2-14C]glucose into the aspartyl moiety of N-acetylaspartic acid (NAA) was conducted. As expected the specific activity of aspartate increased rapidly with time and peaked within 15-20 min after which it fell sharply: but significantly that of the aspartyl moiety of NAA rose very slowly even after the specific activity of aspartate had fallen to less than 1 per cent of the peak values. A rat brain microsomal free supernatant preparation was shown enzymatically to incorporate label from sodium-[l-14C]acetate into the t-RNA fraction from which was isolated N-[1-14C]acetylaspartic acid. From these observations we were inclined to speculate that NAA-t-RNA may serve as an initiator of neuronal protein synthesis

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